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University of Delhi
 
Faculty Member Profile
 
 

ACADEMICS

Prof. Rani Gupta

Prof.
  Professor
     Department of Microbiology, University of Delhi, South Campus
 
     
+919971711806
     ranigupta15@rediffmail.com

Educational Qualifications

Ph.D.

University of Delhi

1983

M.Phil. / M.Tech.

University of Delhi

1979

PG

University of Delhi

1978

UG

University of Delhi

1976

Administrative Assignments

Dean FIAS (2008-2009)

Chairperson, Board of Research Studies, FIAS (2008-2009)

Head, Deptt. Of Biophysics, UDSC, (2008-2009)

Head, Deptt of Microbiology, UDSC (2008-2011)

Member governing committee as University representative in Gargi College (2010-2011) and IHE (2008-2010)

Chairperson, Geetanjali Hostel (2009-2011)

Areas of Specialization / Interest

Industrial Microbiology, Microbial enzymology, Cloning and expression of microbial enzyme and fermentation of industrial important enzyme.

Subjects Taught

Research and teaching experience of more than 25 years

Microbial Physiology and Biochemistry

Eukaryotic microbes

Research Guidance

Supervision of awarded Doctoral Thesis    :  12

Supervision of Doctoral Thesis, under progress   :  03

Supervision of awarded M.Phil dissertations    :   02

 

Publications Profile

Research papers published in Refereed/Peer Reviewed Journals

Gupta, Rani, Richa Sharma, N Apurva and Ratan Murty. 2011. Molecular Characterization of N-Terminal Pro-sequence of Keratinase Ker P from Pseudomonas aeruginosa: Identification of Region with Chaperone Activity. Applied Biochemistry and Biotechnology DOI10.1007/s12010-01.
Gupta, Rani, Rinky Rajput and Richa Sharma. 2011. Cloning and characterization of a thermostable detergent-compatible recombinant keratinase from Bacillus pumilus KS12. Biotechnology and Applied Biochemistry DOI:10.1002/bab.16.
Gupta, Rani and Richa sharma, Rinky gupta. 2010. Biochemical characterization of a thiol activated, oxidation stable keratinase from Bacillus pumilus KS12. Enzyme research DOI:10:4061/2010/132.
Gupta, Rani and Richa sharma. 2010. Extracellular expression of Ker P from Pseudomonas aeruginosa in E.coli. Biotechnology letters. 32: 1863-1868.
Gupta, Rani and Richa Sharma. 2010. Substrate specificity characterization of a thermostable keratinase from Pseudomonas aeruginosa KS-1. Industrial Microbiology and Biotechnology. 37: 785-792.
Gupta, Rani and Ekta Tiwary. 2010. Extracellular expression of keratinase from Bacillus licheniformis ER-15 in Escherichia coli. Journal of agricultural and Food Chemistry. 58: 8380-8385.
Gupta, Rani and Ekta Tiwary. 2010. Subtilisin-γ glutamyl transpeptidase, a novel combination as ungual enhancer for prospective topical application. Journal of Pharmaceutical Sciences. 99: 4866-4873.
Gupta, Rani and Ekta Tiwary. 2010. Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15: Biochemical characterization and application in feather degradation and dehairing of hides. Bioresource technology. 101: 6103-6110.
Gupta, Rani and Ekta Tiwary. 2010. Improved catalytic efficiency of a monomeric γ-glutamyl transpeptidase from Bacillus licheniformis in presence of Subtilisin. Biotechnology letters. 32: 1137-1141.
Gupta, Rani, S Suresh Kumara, Naresh Arorab and Raj Bhatnagar. 2009. Kinetic modulation of Trichosporon asahii MSR 54 lipase in presence of organic solvents: Altered fatty acid specificity and reversal of enantioselectivity during hydrolytic reactions. Journal of Molecular Catalysis B: Enzymatic. 59: 41–46.
Gupta, Rani and S Suresh Kumar. 2009. An extracellular lipase from Trichosporon asahii MSR 54: Medium optimization and enantioselective deacetylation of phenyl ethyl acetate. Process Biochemistry. 43: 1054–1060.
Gupta, Rani, S Suresh Kumar, Lalit Kumar and Vikram Sahai. 2009. A thiol-activated lipase from Trichosporon asahii MSR 54: Detergent compatibility and presoak formulation for oil removal from soiled cloth at ambient temperature. J Ind Microbiol Biotechnol. 36: 427–432.
Gupta, Rani and P Ramnani. 2007. Keratinase vis--vis conventional proteases and feather degradation. World Journal of Microbiology and Biotechnology. 23: 1537-1540.
Gupta, Rani, N Gupta and V Sahai. 2007. Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochemistry. 42: 518-526.
Gupta, Rani R Singh, N Gupta and V Goswami. 2006. A simple activity staining protocol for lipases and esterases. Applied Microbiology and Biotechnology. 70: 679-682.

CONFERENCE PARTICIPATION/PRESENTATIONS

Organization of a Conference

4th Congress of European Microbiologists (FEMS 2011) held at Geneva, Switzerland 2011.

International conference on Microorganisms in environmental management and Biotechnology held at Bhopal, 2011.

108th meeting of American Society of Microbiology held at Boston, USA 2008

An international conference on Biotechnology for Sustainable Resources, NCL Pune, National Chemical Laboratory, 2008

3rd BRSI convention and international conference on exloring horizons in biotechnology: A Global Venture held in Vallabh Vidyanagar, 2006.

Association of Microbiologist of India held in Bhopal, 2006.

Participation as Paper/Poster Presenter

Presentation on “Purification and Kinetic comparison of three forms of γ-glutamyl transpeptidase isolated from fermentation broth of Bacillus licheniformis ER-15” in International conference on “108th Annual conference of American Society of Microbiology” held at Boston, USA. June 1st -5th, 2008.

Presentation on “Cloning and Expression of Subtilisin stable γ-glutamyl transpeptidase” National conference on “49th Annual AMI Conference” held at New Delhi, India. November 18th-20th, 2008.

Presentation on “Keratinase from Pseudomonas aeruginosa KS-1 structural and biochemical basis of feather degradation” National conference on “49th Annual AMI Conference” held at New Delhi, India. November 18th-20th, 2008.

Presentation on “Lipase from Burkholderia multivorance: Biochemical characterization and gene sequence analysis” in International conference on “Applied and Environmental Microbiology” held at Connecticut, USA. July 24th- 29, 2005.

Presentation on “Cloning of an alkaline -keratinase from Bacillus licheniformis RG1 and its expression in E.coli” in International conference on “Applied and Environmental Microbiology” held at Connecticut, USA. July 24th- 29, 2005.

Presentation on “Enzymatic fat splitting of mustard oil for extraction of major fatty acids” in conference on “ Resource development and marketing issues in rapeseed- mustard” held at National Institute of agricultural marketing (NIAM), Jaipur, Rajasthan. March 28th- 29, 2005

Attended and was also a part of organizing committee of International conference on “Microbial diversity: Current perspectives and potential applications” held at Department of Microbiology, University of Delhi South Campus, New Delhi. , 2005

Attended Workshop on “Bioinformatics 2003” at JNU, New Delhi –110067

Presentation on “Production and characterization of a thermostable alkaline esterase from Pseudomonas sp.” in “Annual conference of Association of Microbiologist of India (AMI)”held at HAU Hissar, Haryana. December 05, 2002.

Thiol-dependent, bleach-stable alkaline serine protease from Bacillus mojavensis: Purification, characterization and applications. Beg Q K and Gupta R. (2002). ESBES-4, 4th European Symposium on Biochemical Engineering Science, Technische Universiteit, Delft, Netherlands, August 28-31, 2002.

Attended and was also a part of organizing committee of International conference “Thermophiles 2001” held at Department of Microbiology, University of Delhi South Campus, New Delhi. December 3-7, 2001.

Protease synthesis in Bacillus mojavensis by de-repression and induction under semi-batch and fed-batch operations. Beg Q K and Gupta R. (2001). National Symposium on Lignocellulose Biotechnology: Present and future prospects. Department of Microbiology, University of Delhi South Campus, New Delhi, December, 10-11, 2001.

Stereoselective acylation of ascorbic acid using hyper-thermostable lipases from Bacillus stearothermophilus and Burkholderia cepacia. Rathi P, Saxena RK and Gupta R. (2001). Biotrans 2001, The 5th International Symposium on Biocatalysis and Biotransformations, Techsche Universitat, Darmstadt, Germany. Sep. 2-7, 2001.

Enhanced protease synthesis in Bacillus mojavensis by de-repression and induction under fed-batch operations. Beg QK, Saxena RK and Gupta R. (2001).  Biotrans 2001, The 5th International Symposium on Biocatalysis and Biotransformations, Techsche Universitat, Darmstadt, Germany. Sep. 2-7, 2001.

Bleach-stable alkaline proteases from Microorganisms. Qasim Khalil Beg,    Ruchi Oberoi, R.K. Saxena and Rani Gupta. (2000). Annual Conference of All India Institute of Chemical Engineers (Chemcon 2000), Calcutta.

Control of protease production from Bacillus sp. under de-repression and subsequent induction. Qasim Khalil Beg, R.K. Saxena and Rani Gupta. (2000). Annual Conference of Microbiologists of India. Birla Institute, Jaipur.

Purification and characterization of a novel hyperthermostable, acidic lipase from Bacillus sterarothermophilus SB-1. Pooja Rathi, Sapna Bradoo, R.K. Saxena and Rani Gupta. (2000). Annual Conference of Microbiologists of India. Birla Institute, Jaipur.

Reduce uptake and efflux as mechanism of zinc tolerance in Oscillatoria  anguistissima. Prerna Ahuja, Harapriya Mohapatra, R. K. Saxena and Rani Gupta (2000).  Annual Conference of Microbiologists of India. Birla Institute, Jaipur.

Production and characterization of a novel hyperthermostable alkaline lipase from Pseudomonas sp.  Pooja Rathi, RK. Saxena and Rani Gupta (2000). International Conference Micorbial Biotechnology, trade and public policies. Osmania University, Hyderabad.

Partitioning and resolution of mixture of two lipases from Bacillus stearothermophilus SB- 1 in aqueous two-phase system. Sapna Bradoo, R.K.Saxena and Rani Gupta (1999). First University-Industry Interaction meet on Lipase research: needs and components, University of Delhi South Campus, Delhi.

High yields of ascorbyl palmitate by lipase mediated esterification. Sapna Bradoo, R.K.Saxena and Rani Gupta (1999). First University-Industry Interaction meet on Lipase research: needs and components, University of Delhi South Campus, Delhi.

Paper presented on ‘Oscillatoria anguistissima: A promising biosorbent for removing heavy metals from waste water’. Mohapatra, H. and Gupta R. In the seminar on Venture Agrobiotechnology held at Thapar Centre for Industrial Research & Development, Patiala (September 24-25, 1999).

Lipase mediated synthesis of oil soluble vitamin C conjugates for food and personal care products. Sapna Bradoo, R.K.Saxena and Rani Gupta (1998). 39th Annual Conference of Association of Microbiologists of India held at Mangalore.

Separation and purification of two lipases from Pseudomonas sp. In aqueous two phase systems by a three step extraction system Rani Gupta, Sapna Bradoo, R.K.Saxena, Sheba Davidson and Ruchi Gulati (1998) National Seminar on Bioprocessing and recombinant-DNA technology, CDRI, Lucknow

A rapid scheme for production and down stream processing of lipase from Aspergillus terreus. Ruchi Gulati, R.K.Saxena, Rani Gupta, Sheba Davidson, Sapna Bradoo (1998). National Seminar on Bioprocessing and recombinant-DNA technology, CDRI, Lucknow.

A constitutive alkaline protease from Bacillus sp. for detergent application. Seema Khan, Rani Gupta and R.K.Saxena. National Seminar on Prespectives in Interfacial Areas of Chemistry and Biology (1998), Delhi India.

Partitioning of two lipases from Pseudomonas sp. in Aqueous two phase systems. Rani Gupta, Sapna Bradoo, R.K.Saxena, Sheba Davidson and Ruchi Gulati. National Seminar on Prespectives in Interfacial Areas of Chemistry and Biology (1998), Delhi India.

Partition behaviour and purification of a lipase from A.terreus  using Aqueous two phase system. Ruchi Gulati, R.K.Saxena, Rani Gupta, Sheba Davidson and Sapna Bradoo. National Seminar on Prespectives in Interfacial Areas of Chemistry and Biology (1998), Delhi India.

Synthesis of novel drugs using lipase from Aspergillus carneus. Sheba Davidson, R.K.Saxena, Rani Gupta, Sapna Bradoo, Ruchi Gulati, A. Kumar, H.N.Pati and V.S.Parmar (1998). National Seminar on Perspectives on interfacial areas of Chemistry and Biology.Delhi, India

Interaction of Mycohos and VAM: Effect on P-uptake and Biomass of wheat. Neeraj K.Saxena, Rani Gupta and R.K.Saxena (1997). 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Removal and recovery of heavy metals from aqueous solutions by Oscillatoria  anguistissima. Prerna Ahuja, Rani Gupta and R.K.Saxena. 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

 A constitutive alkaline protease from Bacillus sp. for detergent application. Seema Khan, Rani Gupta and R.K.Saxena (1997). 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Accumulation of copper by Trichoderma viride and its application in effluent treatment. Purnima Anand, R.K. Saxena and Rani Gupta (1997). 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Synthesis of novel drugs using lipase from Aspergillus carneus. Sheba Davidson, R.K.Saxena, Rani Gupta, Sapna Bradoo, Ruchi Gulati, A. Kumar, H.N.Pati and V.S.Parmar (1997) 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Partition behaviour and purification of a lipase from A.terreus using aqueous two phase system. Ruchi Gulati, R.K.Saxena, Rani Gupta, Sheba Davidson and Sapna Bradoo (1997). 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Partitioning of two lipases from Pseudomonas sp. in Aqueous two phase systems. Rani Gupta, Sapna Bradoo, R.K.Saxena, Sheba Davidson and Ruchi Gulati (1997).38th Annual Conference of Association of Microbiologists of India, Delhi, India.

pH dependent production of two lipases from a newly isolated Pseudomonas sp. Sapna Bradoo, Rani Gupta, R.K.Saxena, Sheba Davidson and Ruchi Gulati (1997). 38th Annual Conference of Association of Microbiologists of India, Delhi, India.

Partition behaviour and purification of an acidic lipase from Pseudomonas in Aqueous two phase system. Sapna Bradoo, Rani Gupta, R.K.Saxena, Sheba Davidson and Ruchi Gulati (1997). 10th International Conference on Partitioning in Aqueous Two-Phase Systems, University of Reading, England.

Industrially important thermostable lipases from bacteria. Sapna Bradoo, Ruchi Gulati, Sheba Davidson, Rani Gupta and R.K.Saxena (1996). 37th Annual Conference of Association of Microbiologists of India, Chennai, India.

Low temperature lipases for cold-wash detergents. Ruchi Gulati, Sapna Bradoo,Sheba Davidson, R.K.Saxena and Rani Gupta (1996). 37th Annual Conference of Association of Microbiologists of India, Chennai, India.

Oleic acid in the preservation of cakes. Sheba Davidson, R.K.Saxena and Rani Gupta (1996). International symposium on "Microbiological safety of processed foods", Bangalore, India.

An antistaling enzyme from microbes for baked products. Aditi Ahuja, Rani Gupta, R.K. Saxena and Paresh Gigras (1996). International symposium on "Microbiological safety of processed foods", Bangalore, India.

A potential copper biosorbant and its role in bioremediation. Purnima Anand, Prerna Ahuja, R.K.Saxena and Rani Gupta (1996). National Symposium on current trends in biochemistry and biotechnology of fungi, Hyderabad, India.

An industrially important alkaline lipase from A.carneus. Sheba Davidson,  R.K.Saxena, Rani Gupta, Ruchi Gulati and Sapna Bradoo. (1996). National Symposium on current trends in biochemistry and biotechnology of fungi,Hyderabad, India.

The catalytic potential of Aspergillus carneus lipase.Sheba Davidson, R.K.Saxena and Rani Gupta (1996). Thermophiles'96, University of Georgia, Athens, Georgia, U.S.A.

 Bioleaching of copper from ferromanganese sea nodule of Indian Ocean by natural isolates of Aspergillus spp. Seema Khan, Rani Gupta and R.K.Saxena (1996). Recent Trends in Biological treatment of industrial wastes. MACS-ARI, Pune, India.

Copper removal through biosorption by Oscillatoria sp. Prerna Ahuja, Rani Gupta and R.K.Saxena 1995). XXXVI Annual Conference of Association of Microbiologists of India. CCS, HAU, Hissar, India

Mechanism of bioleaching of copper from ferromanganese sea nodules of Indian Ocean by Aspergillus foetidus and Aspergillus japonicus. Seema Khan, Rani Gupta and R.K. Saxena (1995). 64th Annual Meeting of Society of Biological Chemists (India) Lucknow, India. (P2C10) pp.155.

A rapid procedure for partial purification of extracellular tannase for industrial purpose. Sapna Bradoo, Rani Gupta and R.K.Saxena (1995). 64th Annual Meeting of Society of Biological Chemists (India), Lucknow, India (P1C 22) pp.49.

Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus.Sapna Bradoo, Rani Gupta and R.K.Saxena (1995). XXXVI Annual Conference of Association of Microbiologists of India, CCS, HAU, Hissar, India (IM-7) pp.162.

Catalytic potential of Aspergillus carneus lipase and future vision for organic synthesis. W. Sheba Davidson, R.K.Saxena, R.P.Yadav and Rani Gupta (1995). XXXVI Annual Conference of Association of Microbiologists of India. CCS, HAU, Hissar, India (IM-36) pp.177-178.

A rapid plate assay for screening L-asparaginase producing microorganisms. Ruchi Gulati, R.K.Saxena and Rani Gupta. (1995). XXXVI Annual Conference of Association of Microbiologists of India, CCS, HAU, Hissar, India (IM-29) pp.174.

Physico-chemical guidance of lipase activity in aqueous system and its possible active site model. Yadav, R.P., R.K.Saxena and Rani Gupta (1994). 16th International Congress of Biochemistry and Molecular Biology, New Delhi, India, pp.226.

Regulation of an abbreviated, synchronized developmental process - microcycle differentiation. Nitika Khurana, R.K.Saxena and Rani Gupta (1994). 16th International Congress of Biochemistry and Molecular Biology, New Delhi, India, pp.81.

Metal accumulatioin of microorganisms - A new perspective for biotechnology. Purnima Anand, Prerna Ahuja, Rani Gupta and R.K.Saxena (1994). 16th International Congress of Biochemistry and Molecular Biology, New Delhi, India, pp.150.

Solvent engineering for lipase catalysed trasnesterification alternative to protein engineering. R.P. Yadav, R.K.Saxena and Rani Gupta (1994). National seminar on application of biotechnology in molasses based and allied industries trends and prospects (Abst PP-10) 50.

Solubilization of different native inorganic phosphates and low grade rock phosphates by some soil fungi. Rekha Singal, Rani Gupta and R.K.Saxena (1994). XXXIV Annual Conference of Association of Microbiologist of India (Abst-SM73) pp.158.

Tolerance and accumulation of heavy metal in cynobacteria. Prerna Ahuja, Rani Gupta and R.K.Saxena (1994). XXXIV Annual Conference of Association of Microbiologist of India (Abst-EM26) 87.

Thermostable lipase from A.terreus. R.P.Yadav, R.K.Saxena and Rani Gupta (1993). 62nd Annual meeting at Society of biological chemists (India) Abst. (PEFDO-39) 205-206.

Bio-economic Production and Standardization of Lipase from A.terreus and its future vision in Biosurfactant Industry. R.P.Yadav, Rani Gupta and R.K.Saxena (1993). XIth National Symposium on ‘Catalysis: Catalysis - Present & Future'.

Thermostable exolipases from Streptomyces sp. Kapil Kumar, R.P.Yadav, Rani Gupta, R.K.Saxena (1992). XXXIIIAnnual Conference of Association of Microbiologists of India.

Chitinase production from Streptomyces viridificans. Priya Chaturvedi, J.S.Virdi, Rani Gupta, R.K.Saxena and R.C.Kuhad (1992).  XXXIII Annual Conference of Association of Microbiologists of India.

Lipase mediated bio-conversion of animal fat into "Green Chemicals" R.P.Yadav, R.K.Saxena and Rani Gupta (1992). XXIII Annual Conference of Association of Microbiologists of India.

Light independent conidiation in Trichoderma spp.: a novel approach to Microcycle conidiation. Nitika Khurana, R.K.Saxena and Rani Gupta (1992).XXXIII Annual Conference of Association of Microbiologists of India.

Induction and regulation of microcycle conidiation of the genus Aspergillus. R.K.Saxena, Nitika Khurana and Rani Gupta (1991). XXXII Annual Conference of Association of Microbiologists of India.

Utilization of Inorganic phosphates by a basidiomycetous fungus Cyathus. Rekha Singhal, Rani Gupta, R.C.Kuhad and R.K.Saxena (1990). Fourth International Mycological Congress. Regensuburg, Germany.

Research Projects (Major Grants/Research Collaboration)

Ongoing Projects

Characterization of ketoreductases from newly isolated yeasts strains: Application in production of enantiopure phenyl ethanol. UGC.

Biochemical and molecular characterization of an enantioselective lipase from Trichosporon asahii MSR 54. CSIR.

Subtilisin-gamma Glutamyl Transpeptidase, a novel biocatalytic enzyme complex for efficient ungual drug delivery: Expression and protein-protein interaction. DU-DST PURSE.

Keratinase from Bacillus pumilus for degradation of recalcitrant proteins: Process optimization and Biochemical characterization. DBT.

Completed

Biosurfactant from newly isolated yeasts strains: Fermentation, downstream processing, characterization  and its application (DBT).

Novel lipases from yeast strains isolated from petroleum sludge: Lipase production, purification and characterization with respect to their catalytic utility (CSIR).

Large scale production of food and feed enzymes: Development of enzyme consortia for use in bakery and feed industry (DBT).

Process development for removal of heavy metals from waste waters using cynobacterial biomass (DBT).

Process optimization for a thermostable lipase from Aspergillus terreus (DBT).

Production and characterization of Intermediate thermostable (ITS) α-amylase for the (EMB) Enzyme Modified Bread technology (DBT).

Mass inoculum production of Ceriporioposis subvermispora and Phanerochaete chrysosporium for biobleaching and biopulping for paper industry (DBT).

Production, purification and characterization of a bleach stable alkaline protease from Bacillus sp. and its application in detergent (CSIR).

Cloning and over expression of novel thermostable bacterial lipases and their applications in biotransformation (DST-DAAD).

Production and characterization of protease and lipase enzymes from Bacillus sp. for their commercial applications (International Panacea Ltd.)

Concomitant production of a protease and Biosurfactant by a Bacillus sp. and their potential applications as bread improvers (ICAR).

Hyper produced lipase from Burkholderia sp. production characterization and application in esterification and transesterification (DBT).

Cloning and expression of a bleach stable alkaline protease from Bacillus sp. (CSIR).

Microbial proteases and lipases for the production of food related value added products (Ministry of food processing industries).

Awards and distinctions

Pool officership: May 1984 & Jan. 1987.

National Associateship of DBT, Government of India (1997) worked at I.I.T., New Delhi, India

Association with Professional Bodies

Reviewing  industrial         

Process Biochemistry, Applied Microbiology and Biotechnology

Journal of Microbiology and Biotechnology

Bioresource technology

Indian journal of Microbiology and other related microbiology and biotechnology journals 

Memberships              

AMI, Life member, ASM, annual member